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DNA Primers Explained

Written by: The Midland Certified Reagent Company

Summary: Primers help initiate reactions that are crucial to modern medicine.

When researchers want to test a cure, they are hoping for a particular reaction. That reaction will tell them what they need to know about how the cure might react to a patient’s DNA. It may also tell doctors what kind of diseases a patient may have, or potentially develop.

These reactions don’t just occur, there needs to be some kind of primer. A polynucleotide can help initiate a reaction through the binding of complementary pairs. That initial molecule that sparks the reaction is called a DNA primer.

What are Primers?

Primers are like shorter sequences of nucleic acids, usually comprising only around 10 base pairs. When synthesis occurs naturally, the body relies on a short strand of RNA to replicate. Primers add extra pairs to a DNA strand, which helps catalyze a bond.

Each primer is hybridized for a specific pairing, so the reactions are possible only under ideal conditions.

Uses

Primers find many uses in DNA sequencing. Primers are used to begin the Sanger chain termination reaction, which is still used today as a method of verifying next-generation calculations as accurate. Primers need to match the DNA molecules they are meant to amplify, and they are used in a polymerase chain reaction as a kind of probe that helps identify which DNA fragments were changed by the PCR.

Final Thoughts

Primers make a wide variety of reactions possible. They are useful in diagnosis, but helpful in research too. Primers are sometimes used for RNA synthesis, not just for DNA synthesis.


 

Bio: The Midland Certified Reagent Company produces poly dI and poly C oligonucleotides, as well as other materials used in research labs around the world.

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Keep diabetes under control with green tea

Article written by Cellan.

Diabetes affects many and more than 300 million people worldwide suffer from type II diabetes. It helps to create elevated levels of blood sugar resisting insulin or contributes to one’s inability to produce insulin. This is why many are injecting insulin daily. Those who are suffering from type II diabetes, green tea can help by improving insulin sensitivity and by reducing blood sugar levels.

One thing that is clear is green tea has been used for centuries to control blood sugar. Researchers find that green tea helps to prevent type I diabetes and keep blood sugar level in check. Those who are suffering from type I diabetes lose their ability to produce insulin, a hormone that is needed to usher glucose into cells. EGCG helps to regulate glucose due to its inhabitation of carbohydrate digesting enzymes. Those who are with diabetes carry the increased risk of developing cardiovascular disease and require higher doses of antioxidants to fight it. Cellan Diet contains 100 percent green tea extract to give you the benefits of EGCG.


Recent research links green tea and its EGCG to help control blood sugar levels, keep type I and type II diabetes in check and help with insulin levels. This is why Cellan Diet Pills contain green tea extract as one of its main ingredients.

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Simple Trick to Combat Negativity

Written by Heather Richards

Are you determined to stay positive no matter what life throws your way? People who are optimistic tend to be happier, healthier, and more open to the bounty that life has to offer. While there are many reasons to stay positive, life doesn’t always give us the best environment to foster our positive attitudes.

Sometimes the people around us can bring negativity. The things they say or do can cause us to doubt our own dreams and desires. So how do you stop the pessimistic people in your life from poisoning your dreams? It might be easier than you think. Dr. Eric Amidi offers the following trick to help people combat negativity:

Do not fear negativity. According to Dr. Amidi, before you can stop negative people from bringing you down it’s important to stop fearing it. Dr. Eric Amidi says that the fear of negativity will actually attract more of it in your life. This is because your subconscious gets the message that you’re afraid of it and will see it as a threat or obstacle to getting what you want in life.

So how do you stop being afraid of negativity? By not fighting it. Instead, recognize it, neutralize it, and “turn it into positivity.” This means that it’s important to first detect the negative feelings or thoughts. Instead of denying them or trying to block them out of your mind, accept them for what they are. Next, get ready to bring positivity into your life. To learn more how you can prevent negativity from dampening your dreams, follow the writings of Dr. Eric Amidi.

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Miracle fruit to help you reduce bad cholesterol

Article written by Cellan. Miracle super fruit African mango is used in food preparations and others but the most important part is its seed. Researchers find that inner seeds to help lower bad cholesterol without interfering with cholesterol reducing medications. This is why Cellan Diet Pills contain dried and powdered inner seeds of African mango.

Known to locals as Dikka fruit, African mango is considered a miracle fruit by people around the world. Its flesh has been used as fruit and to thicken soup and other food preparations. But the most valuable part of the fruit is its inner seed. Recent research indicates that the seed brings many benefits including healthy fats, fiber, help reduce bad cholesterol in humans, improve hormone adiponectin, and results in weight loss.

Using seeds directly or supplements such as Cellan Diet Pills that contain dried and powdered inner seeds helps to improve heart health and reduce bad cholesterol over the long term. Researchers also find that the seeds do not interfere with drugs that are used to reduce triglycerides and low-density lipoprotein (LDL). This is good news for many who suffer from an elevated level of bad cholesterol.

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Valuable Tips on How to Achieve Your Goals

goals-jump

Written by Heather Richards

Most of us have goals, whether it is losing weight, getting a better job, or graduating from college. Some of us want to start a family, while others want to learn how to cook. Unfortunately, most of us lose steam and can’t focus on our goals long enough to achieve the things we want in life. This is especially true for long-term goals.

But there are ways you can stay focused and get the desires of your heart. If you’re having trouble reaching your goals, you’re not alone. Here are a few tips on how to set and reach your goals:

Focus on One Goal at a Time

Many of us have multiple plans in our minds at the same time. We set our goals and try to find time and energy for each one, always balancing various plans. But there is another method we can use that helps us reach our goals faster. This method is focusing on one goal at a time.

Dr. Eric Amidi often writes about this strategy and says that we should focus on one desire or goal first. In a recent blog post the quantum physicist uses the example of the cheetah’s hunting technique to illustrate his point. He says that a cheetah has the ability to focus on a single gazelle and will use all of its energy to run after and capture that one gazelle, even if he runs next to other gazelles during the chase. Dr. Eric Amidi adds that chances are you will learn a lot about yourself by reaching the first goal and you can use the insight to help you reach the next one.

Enjoy the Process

One of the problems with setting goals is that a goal is not realized until a certain time in the future, it’s a place you haven’t reached yet. For this reason many people who set goals are simply not happy or satisfied until they’ve reached their goals. It is this unhappiness that causes many to give up on their dreams.

The key to staying happy until you reach your goal is to enjoy the journey or process along the way. According to an article in Psychology Today written by James Clear, the solution is to “commit to a process, not a goal.” He writes that many people feel as though they’re not good enough until they reach their goal, but this is just not the case. With this mindset, people avoid feeling happy until “the next milestone is achieved.”

This point of view is further explored by Dr. Eric Amidi who also agrees that we should not separate our goals from the process or journey it takes to achieve them. He suggests that the goal and the process are the same.

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Flavonoids to provide the fighting power against many conditions

Article written by Cellan Diet Pills. Green tea is rich in flavonoids that are anti-oxidant, anti-allergy, anti-inflammatory, anti-bacterial, anti-viral and even anti-cancer. This is why Cellan Diet contains 100 percent green tea extract as one of its main ingredients.

According to stories circulating on the Internet, Chinese Oolong tea was discovered by accident by a person named Wu Liang some 400 years ago. Ever since the discovery, Chinese have been using tea for many reasons especially for its well-proven health benefits. Recent studies prove that tea brings certain benefits. It is a natural source of flavonoids that provide defense against oxidants, allergies, inflammation, bacterial, viral and even cancer. The amount of flavonoids in tea is determined by its process. If more processing including fermentation is involved, then the flavonoids levels decreases. This is why black tea contains less flavonoid than green tea. This is why less processed green tea is full of flavonoids. Its main ingredient that provides the fighting power is known as EGCG (epigallocatechin 3-gallate). This is why Cellan contains 100 percent green tea extract as one of its main ingredients to give you the fighting power against many conditions.

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The Experimental Nature of RNA Synthesis

Written by: The Midland Certified Reagent Company

Summary: Synthetic DNA allows scientists to recreate certain sequences within a lab to test how they might fight disease.

DNA, the building block of life, comes configured with extremely specific traits that define everything about life forms. DNA synthesis allows scientists to manipulate those building blocks in specific combinations. Most people think “designer baby” when they hear this concept for the first time, but there are important medical research studies that require specific DNA sequences in order to test cures and remedies.

Synthesis

Synthesis is a natural process. DNA and RNA synthesis occurs within the body to form new proteins and new DNA molecules. Cracking the genome has enabled scientists to view disease and disorders in a whole new light. Combined with artificial synthesis, scientists are able to study very specific conditions that are related to diseases.

Oligo Synthesis

This process must be extremely precise, and is therefore automated. Oligonucleotide synthesis only deals with a defined chemical sequence, but this can be an advantage. Creating an entire DNA polymer is time consuming and expensive. It is still performed today, but oligos synthesis allows scientists to maintain a tight control over a desired sequence.

Uses

When a researcher has a theory about a disease, he or she has to try and recreate those conditions in a laboratory in order to study that theory in further detail. Patients with the disease may exist, but what if the researcher wants to hone in on people with a specific strand of DNA? In these situations, it’s not always feasible to track down patients that match the specific criteria. Synthetic DNA can act as a springboard, so to speak, giving the research team something they can work with to cure a disease.

Dr. Eric Amidi Reveals Trick to Staying Positive

Written by Heather Richards

By now most of us know the importance of staying positive. A positive outlook on life not only brings you happiness but also blesses the people around you. Someone who is positive is optimistic, always focusing on the good things in life instead of dwelling on the bad. But remaining positive on a regular basis can be challenging, especially if you live or work with people who try to bring you down. Whether you’re experiencing a storm in your life or dealing with negative people, it’s still possible to stay positive. In fact, some people even believe it’s the key to getting what you want and need in this life.

In a recent blog post titled How to Switch to Positivity, Dr. Eric Amidi unveils a simple trick on how to stay positive despite being surrounded by negative people. (Dr. Amidi is the author of the ebook “The Secret Behind the Secret,” and he often writes about the Law of Attraction.) Part of what he shares in the blog is something I struggle with: the fear of negativity. Dr. Eric Amidi writes that it’s important not to fight or fear negativity. Instead, he writes, recognize it and learn how to “neutralize it and turn it into positivity.” According to Dr. Amidi, you must first master positivity within yourself before you can block the negativity of others or stop it from bringing you down.

So what’s his trick? Read his blog post to find out what Dr. Eric Amidi says. I will, however, reveal that it’s as easy as asking yourself or another person one question. Yup. Just one.

 

 

Dr. Eric Amidi writes, advises, and consults on productivity, entrepreneurship, and life-transforming self-improvement methods.

 

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Combined power of African mango and green tea

Article written by Cellan Diet. African mango combined with green tea becomes a powerful weapon against body weight, blood sugar, fat and belly fats. Cellan contains both ingredients as well as other proven weight loss agents.

Any weight loss program should aim at not just loosing body weight but also loosing body fat and size of a person’s waist line that indicates his or her belly fat. Studies conducted for effectiveness of African mango and EGCG found in green tea reveals exiting results on weight loss. Combining the powers of both ingredients to bring down the body weight as well as lower blood sugar and “bad” cholesterol reveals that together they are a powerful combination to lose body weight. In order to achieve benefits of both ingredients, one should also follow a calorie controlled diet and embark on a regular exercise program. Together, they may help you to lose 25 to 30 pounds, lower waist size by six inches, increase your metabolism, and control your craving for food. This is why supplements such as Cellan Diet Pills contain dried and powdered inner seeds of African mango, 100 green tea extract and other proven fat and weight loss ingredients.

Get More Results By Taking It Easy

Written by Dr. Eric Amidi.

When we talk about putting “effort” into the manifestation process, it’s easy to think that we mean the type of hard work that you would associate with a desk job. This is far from the reality. The ability to focus your energy to get positive things to happen for you does require effort, but it’s far different than the “hard work” that you might associate with your job. In fact, it’s a paradox: the harder you work, the more likely it is that you’ll have to work even harder to achieve your success.

The most effective way to achieve success in the manifestation process is to impress positive influences on your subconscious mind which is not something you can simply do through force of will. In fact, your subconscious mind will often fight against attempts to influence it through maximum effort. Instead, you need to work a different approach and influence your subconscious through feelings.

Scarcity is driven by the feeling of trying too hard for something — you might even call it desperation. Consider what happens if someone is trying too hard to make a relationship work or to get hired for a job. Chances are that they won’t be successful.

In order to impress positive feelings on your subconscious mind, you have to fight against the urge to solve the problem through exertion. Instead, you need to let your subconscious become taken by the powerful feelings of positive energy that you are creating. This will lead to feelings of abundance that can only mean great things for the rest of your life.

Eric Amidi is a best-selling author who has used his scientific background to help people better understand the relationship between their thoughts and their success. Dr. Eric Amidi is a physicist who was part of the team that discovered the Top Quark.